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mouse monoclonal twin strep tag primary antibody  (IBA Lifesciences)


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    Structured Review

    IBA Lifesciences mouse monoclonal twin strep tag primary antibody
    Mouse Monoclonal Twin Strep Tag Primary Antibody, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 96/100, based on 325 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+strep/StrepMAB-Classic/pm42045187-272-5-12
    Average 96 stars, based on 325 article reviews
    mouse monoclonal twin strep tag primary antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Niche Tet maintains germline stem cells independently of dioxygenase activity.
    Article Snippet: Ten-eleven translocation (TET) proteins are dioxygenases that convert 5-methylcytosine (5mC) into 5-hydroxylmethylcytosine (5hmC) in DNA and RNA.. However, their involvement in adult stem cell regulation remains unclear.. Here, we identify a novel enzymatic activity-independent function of Tet in the Drosophila germline stem cell (GSC) niche.

    Article Title: Niche Tet maintains germline stem cells independently of dioxygenase activity
    Article Snippet: .. For pull-down and western blotting, mouse anti-HA (1:2000, Sigma-Aldrich, H3663), mouse anti-Myc (1:2000, Sigma-Aldrich, M5546), or mouse anti-Strep (1:2000, IBA Lifesciences, 2-1507-001) antibodies were employed. ..

    Saline:

    Article Title: Structure of the measles virus ternary polymerase complex.
    Article Snippet: Proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, IPVH00010). .. The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h, followed by incubation with primary antibodies at 4 °C overnight: rabbit anti-HA (1:2000, Cell Signaling Technology, C29F4), mouse anti-Flag (1:2000, Sigma, F1804), mouse anti-His (1:3000, Invitrogen, His.H8, MA1-21315), and mouse anti-Strep (1:1000, IBA, 2-1507- 001). .. Detection of the primary antibodies was carried out using HRPconjugated secondary antibodies (Cell Signaling Technology), including goat anti-rabbit (7074S) and rabbit anti-mouse (58802S), each at 1:5000.

    Article Title: Structural basis of measles virus polymerase inhibition by nonnucleoside inhibitor ERDRP-0519
    Article Snippet: For Western blot analysis, the lysate supernatant was denatured in 2× sample loading buffer, boiled at 100 °C for 5 minutes, separated by SDS-PAGE, and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, IPVH00010). .. Membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h, followed by incubation with primary antibodies at 4 °C overnight: rabbit anti-HA (1:2000, Cell Signaling Technology, C29F4), mouse anti-Flag (1:2000, Sigma, F1804), mouse anti-His (1:3000, Invitrogen, His.H8, MA1-21315), and mouse anti-Strep (1:1000, IBA, 2-1507-001). .. Detection of the primary antibodies was carried out using HRP-conjugated secondary antibodies (Cell Signaling Technology), including goat anti-rabbit (7074S) and rabbit anti-mouse (58802S), each at 1:5000.

    Article Title: Structure of the measles virus ternary polymerase complex
    Article Snippet: Proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, IPVH00010). .. The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h, followed by incubation with primary antibodies at 4 °C overnight: rabbit anti-HA (1:2000, Cell Signaling Technology, C29F4), mouse anti-Flag (1:2000, Sigma, F1804), mouse anti-His (1:3000, Invitrogen, His.H8, MA1-21315), and mouse anti-Strep (1:1000, IBA, 2-1507-001). .. Detection of the primary antibodies was carried out using HRP-conjugated secondary antibodies (Cell Signaling Technology), including goat anti-rabbit (7074S) and rabbit anti-mouse (58802S), each at 1:5000.

    Article Title: Structural basis of measles virus polymerase inhibition by nonnucleoside inhibitor ERDRP-0519.
    Article Snippet: For Western blot analysis, the lysate supernatant was denatured in 2× sample loading buffer, boiled at 100 °C for 5minutes, separated by SDSPAGE, and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, IPVH00010). .. Membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h, followed by incubation with primary antibodies at 4 °C overnight: rabbit anti-HA (1:2000, Cell Signaling Technology, C29F4), mouse anti-Flag (1:2000, Sigma, F1804), mouse anti-His (1:3000, Invitrogen, His.H8, MA1-21315), and mouse anti-Strep (1:1000, IBA, 2-1507-001). .. Detection of the primary antibodies was carried out using HRP-conjugated secondary antibodies (Cell Signaling Technology), including goat anti-rabbit (7074S) and rabbit anti-mouse (58802S), each at 1:5000.

    Incubation:

    Article Title: Structure of the measles virus ternary polymerase complex.
    Article Snippet: Proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, IPVH00010). .. The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h, followed by incubation with primary antibodies at 4 °C overnight: rabbit anti-HA (1:2000, Cell Signaling Technology, C29F4), mouse anti-Flag (1:2000, Sigma, F1804), mouse anti-His (1:3000, Invitrogen, His.H8, MA1-21315), and mouse anti-Strep (1:1000, IBA, 2-1507- 001). .. Detection of the primary antibodies was carried out using HRPconjugated secondary antibodies (Cell Signaling Technology), including goat anti-rabbit (7074S) and rabbit anti-mouse (58802S), each at 1:5000.

    Article Title: Structural basis of measles virus polymerase inhibition by nonnucleoside inhibitor ERDRP-0519
    Article Snippet: For Western blot analysis, the lysate supernatant was denatured in 2× sample loading buffer, boiled at 100 °C for 5 minutes, separated by SDS-PAGE, and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, IPVH00010). .. Membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h, followed by incubation with primary antibodies at 4 °C overnight: rabbit anti-HA (1:2000, Cell Signaling Technology, C29F4), mouse anti-Flag (1:2000, Sigma, F1804), mouse anti-His (1:3000, Invitrogen, His.H8, MA1-21315), and mouse anti-Strep (1:1000, IBA, 2-1507-001). .. Detection of the primary antibodies was carried out using HRP-conjugated secondary antibodies (Cell Signaling Technology), including goat anti-rabbit (7074S) and rabbit anti-mouse (58802S), each at 1:5000.

    Article Title: Mycobacterium tuberculosis long-chain fatty acid resistome reveals universal stress protein TB15.3 as essential for infection.
    Article Snippet: .. After transfer and blocking, membrane was cut between the 30 and 37 kDa marker and the top part was incubated for 1.5h at RT with Mouse Anti-Strep (1:2,000) (iba, 2-1507-001) primary antibody to stain for Strep_Dxs2 (~75 kDa), whereas the bottom membrane was incubated with Mouse Anti-His (1:20,000) (GenScript, A00186) for 1h at RT to stain for His_TB15.3 (~16 kDa). .. Both membranes were incubated together with the secondary antibody Rat Anti-mouse IgG HRP (1:5,000) (Sigma, A4416) for 30 min at RT.

    Article Title: Structure of the measles virus ternary polymerase complex
    Article Snippet: Proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, IPVH00010). .. The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h, followed by incubation with primary antibodies at 4 °C overnight: rabbit anti-HA (1:2000, Cell Signaling Technology, C29F4), mouse anti-Flag (1:2000, Sigma, F1804), mouse anti-His (1:3000, Invitrogen, His.H8, MA1-21315), and mouse anti-Strep (1:1000, IBA, 2-1507-001). .. Detection of the primary antibodies was carried out using HRP-conjugated secondary antibodies (Cell Signaling Technology), including goat anti-rabbit (7074S) and rabbit anti-mouse (58802S), each at 1:5000.

    Article Title: Structural basis of measles virus polymerase inhibition by nonnucleoside inhibitor ERDRP-0519.
    Article Snippet: For Western blot analysis, the lysate supernatant was denatured in 2× sample loading buffer, boiled at 100 °C for 5minutes, separated by SDSPAGE, and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, IPVH00010). .. Membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h, followed by incubation with primary antibodies at 4 °C overnight: rabbit anti-HA (1:2000, Cell Signaling Technology, C29F4), mouse anti-Flag (1:2000, Sigma, F1804), mouse anti-His (1:3000, Invitrogen, His.H8, MA1-21315), and mouse anti-Strep (1:1000, IBA, 2-1507-001). .. Detection of the primary antibodies was carried out using HRP-conjugated secondary antibodies (Cell Signaling Technology), including goat anti-rabbit (7074S) and rabbit anti-mouse (58802S), each at 1:5000.

    Blocking Assay:

    Article Title: Mycobacterium tuberculosis long-chain fatty acid resistome reveals universal stress protein TB15.3 as essential for infection.
    Article Snippet: .. After transfer and blocking, membrane was cut between the 30 and 37 kDa marker and the top part was incubated for 1.5h at RT with Mouse Anti-Strep (1:2,000) (iba, 2-1507-001) primary antibody to stain for Strep_Dxs2 (~75 kDa), whereas the bottom membrane was incubated with Mouse Anti-His (1:20,000) (GenScript, A00186) for 1h at RT to stain for His_TB15.3 (~16 kDa). .. Both membranes were incubated together with the secondary antibody Rat Anti-mouse IgG HRP (1:5,000) (Sigma, A4416) for 30 min at RT.

    Membrane:

    Article Title: Mycobacterium tuberculosis long-chain fatty acid resistome reveals universal stress protein TB15.3 as essential for infection.
    Article Snippet: .. After transfer and blocking, membrane was cut between the 30 and 37 kDa marker and the top part was incubated for 1.5h at RT with Mouse Anti-Strep (1:2,000) (iba, 2-1507-001) primary antibody to stain for Strep_Dxs2 (~75 kDa), whereas the bottom membrane was incubated with Mouse Anti-His (1:20,000) (GenScript, A00186) for 1h at RT to stain for His_TB15.3 (~16 kDa). .. Both membranes were incubated together with the secondary antibody Rat Anti-mouse IgG HRP (1:5,000) (Sigma, A4416) for 30 min at RT.

    Marker:

    Article Title: Mycobacterium tuberculosis long-chain fatty acid resistome reveals universal stress protein TB15.3 as essential for infection.
    Article Snippet: .. After transfer and blocking, membrane was cut between the 30 and 37 kDa marker and the top part was incubated for 1.5h at RT with Mouse Anti-Strep (1:2,000) (iba, 2-1507-001) primary antibody to stain for Strep_Dxs2 (~75 kDa), whereas the bottom membrane was incubated with Mouse Anti-His (1:20,000) (GenScript, A00186) for 1h at RT to stain for His_TB15.3 (~16 kDa). .. Both membranes were incubated together with the secondary antibody Rat Anti-mouse IgG HRP (1:5,000) (Sigma, A4416) for 30 min at RT.

    Staining:

    Article Title: Mycobacterium tuberculosis long-chain fatty acid resistome reveals universal stress protein TB15.3 as essential for infection.
    Article Snippet: .. After transfer and blocking, membrane was cut between the 30 and 37 kDa marker and the top part was incubated for 1.5h at RT with Mouse Anti-Strep (1:2,000) (iba, 2-1507-001) primary antibody to stain for Strep_Dxs2 (~75 kDa), whereas the bottom membrane was incubated with Mouse Anti-His (1:20,000) (GenScript, A00186) for 1h at RT to stain for His_TB15.3 (~16 kDa). .. Both membranes were incubated together with the secondary antibody Rat Anti-mouse IgG HRP (1:5,000) (Sigma, A4416) for 30 min at RT.

    other:

    Article Title: UBR5 forms ligand-dependent complexes on chromatin to regulate nuclear hormone receptor stability
    Article Snippet: Mouse anti-strep , IBA lifesciences , Cat# 2-1507-001; RRID:AB_513133.



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